Taking place on Tuesday and Wednesday, it is the latest in a series of strikes to affect the German airline.
The union's announcement comes days after Lufthansa announced record profits for 2023.
Lufthansa said around 100,000 travellers are likely to be affected.
The strike will cover all departures out of Frankfurt airport on Tuesday and all flights out of Munich on Wednesday, from 04:00 (03:00 GMT) to 23:00 (22:00 GMT) local time, the union said.
It will affect both Lufthansa and its short-distance, low-cost subsidiary, Cityline. It is unclear whether Lufthansa's code share partners will also be affected.
The Independent Flight Attendants Organization (UFO) is calling for a 15% pay increase and a €3,000 ($3,280; £2,550) inflation compensation payment for its 18,000 members with Lufthansa and 1,000 members at Cityline.
It said that more than 96% of UFO's members voted in favour of the strike.
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